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| Registros recuperados: 20 | |
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| Sanches,Lilian Aparecida; Gomes,Marcelo da Silva; Teixeira,Rodrigo Hidalgo Friciello; Cunha,Marcos Paulo Vieira; Oliveira,Maria Gabriela Xavier de; Vieira,Mônica Aparecida Midolli; Gomes,Tânia Aparecida Tardelli; Knobl,Terezinha. |
| ABSTRACT Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of São Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes. After isolation, 401 E. coli strains were subjected to multiplex PCR system with amplification of genes eae and bfp... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrheagenic E. coli; Atypical EPEC; STEC; Avian diseases. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400760 |
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| Carvalho,A.F.; Miyashiro,S.; Nassar,A.F.C.; Noda,A.; Gabriel,D.T.; Baldassi,L.. |
| Foram coletados 100 suabes retais e 100 suabes de carcaças bovinas em matadouros do estado de São Paulo, e um total de 326 estirpes de E. coli foram identificadas, sendo 163 de amostras retais e 163 de amostras de carcaça. Todos os isolados submetidos à PCR para detecção dos genes das toxinas Stx1 e Stx2 foram identificados como não-O157 e fenotipados pelo teste da citotoxicidade em células Vero. Das 26 estirpes que apresentaram apenas o gene stx1, das 56 que apresentaram apenas o gene stx2 e das 30 estirpes que apresentaram ambos os genes, 17 (65,4%), 42 (75%) e 22 (73,3%), respectivamente, foram positivas ao teste de citotoxicidade. Não houve diferença estatística entre os três perfis genéticos e na positividade ao teste de citotoxicidade. Os resultados... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Escherichia coli; STEC; Fezes; Carcaça. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400014 |
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| Cerutti,MF; Vieira,TR; Zenato,KS; Werlang,GO; Pissetti,C; Cardoso,M. |
| ABSTRACT The aim of this study was to investigate the presence of shiga toxin-producing Escherichia coli (STEC) and atypical enteropathogenic Escherichia coli (aEPEC) in frozen chicken carcasses sold at stores in southern Brazil. Typical E. coli colonies were enumerated in 246 chicken carcasses, and the presence of stx1, stx2, eae genes was investigated in their rinse liquid and in E. coli strains isolated from those carcasses. Strains of E. coli were also investigated for the presence of bfp gene. A median of 0.6 cfu.g-1(ranging from <0.1 to 242.7 cfu.g-1) of typical E. coli colonies was found in the carcasses. Shiga toxin-encoding genes (stx1 and stx2) were not detected, indicating that the chicken carcasses were negative for STEC. The intimin protein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken meat; Enteropathogenic Escherichia coli; EPEC; Shigatoxigenic Escherichia coli; STEC. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100310 |
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| Bentancor,A; Agostini,A; Rumi,M.V; Degregorio,O.J. |
| El Síndrome Urémico Hemolítico causado por Escherichia coli shigatoxigénico, STEC, es endémico en Argentina. Si bien es una enfermedad de Transmisión Alimentaria, se postula que el contagio persona-persona tiene alto impacto en nuestro país. Dada la estrecha relación personasanimales de compañía en los centros urbanos, esta ruta ha sido evaluada. El objetivo del trabajo es comunicar los factores de riesgo asociados a la portación-infección por STEC en animales de compañía de Buenos Aires, Argentina, proporcionando información útil para evaluar el riesgo de transmisión de este patógeno. Se recolectaron hisopados rectales de 149 gatos y 450 perros desde abril de 2005 a marzo de 2006. Se elaboró una ficha epidemiológica para cada animal. Se realizó... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: STEC; Síndrome Urémico Hemolítico; Factores de riesgo; Gatos; Perros. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982008000100001 |
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| Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne. |
| Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in... |
| Tipo: Text |
Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf |
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| Gonçalves,V.P.; Marin,J.M.. |
| To determine the fate of Shiga toxigenic Escherichia coli (STEC) non-O 157 in composted manure from naturally colonized cattle, fresh manure was obtained from three cows carrying non-O157 STEC strains possessing the stx2 gene. Two composting systems were used: a 0.6m deep cave opened in the soil and an one meter high solid manure heap in a pyramidal architecture. Every day, for the 10 first days, and every five days for a month, one manure sample from three different points in both systems was collected and cultured to determine the presence of E. coli and the presence of the stx 2 gene in the cells. The temperature was verified at each sampling. STEC non-O157 E. coli cells survived for 8, 25 and 30 days at 42, 40 and 38ºC, respectively, in the deep cave... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial reduction; Manure; STEC; Environment; Composting system. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400001 |
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| Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These... |
| Tipo: Text |
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf |
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| Cantarelli,Vlademir; Nagayama,Kenichi; Takahashi,Akira; Honda,Takeshi; Cauduro,Paulo F.; Dias,Cícero A.G.; Mezzari,Adelina; Brodt,Teresa. |
| We describe the isolation of one strain of Shiga toxin-producing Escherichia coli O91:H21 from a child with diarrhea in the city of Porto Alegre, RS, Brazil. Considering the pathogenic potential of STEC, these organisms should be looked for more carefully among our population. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Enterohemorrhagic E. coli; STEC; Enteroaggregative E. coli; Enteropathogenic E. coli; Diarrhea. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400005 |
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| Baliere, Charlotte. |
| The contamination of coastal areas by potentially pathogenic enteric bacteria is of concern for the sustainability of some uses, such as shellfish farming, recreational shellfish harvesting and bathing. The contamination of these environments may occur through the land-spreading of livestock wastes, animal feces deposited on pastures, wastewaters from slaughterhouses or from municipal wastewater treatment plants. The presence of these bacteria in coastal environment may present a potential risk to human health. In fact, shellfish-borne outbreaks or human infections may occur by the consumption of shellfish from contaminated areas or the ingestion of water during bathing in contaminated waters, respectively. To date, few studies focusing on the presence and... |
| Tipo: Text |
Palavras-chave: STEC; EPEC; Coquillages; Diversité; Virulence; Persistance; STEC; EPEC; Shellfish; Diversity; Virulence; Persistence. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00312/42322/41653.pdf |
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| Novais,Rogério Carlos; Chaves,Marcela Cassin; Gonzalez,Alice Gonçalves Martins; Andrade,João Ramos Costa. |
| tRNA genes are known target sites for the integration of pathogenicity islands (PAI) and other genetic elements, such as bacteriophages, into bacterial genome. In most STEC (Shiga toxin-producing Escherichia coli), the PAI called LEE (locus of enterocyte effacement) is related to bacterial virulence and is mostly associated to the tRNA genes selC and pheU. In this work, we first investigated the relationship of LEE with tRNA genes selC and pheU in 43 STEC strains. We found that 28 strains (65%) had a disrupted selC and/or pheU. Three of these strains (637/1, 650/5 and 654/3) were chosen to be submitted to a RAPD-PCR technique modified by the introduction of specific primers (corresponding to the 5'end of genes selC and pheU) into the reaction, which we... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pathogenicity islands; TRNA; STEC; RAPD-PCR; E.coli. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400020 |
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| Bergamini,Alzira Maria Morato; Simões,Marise; Irino,Kinue; Gomes,Tânia Aparecida Tardelli; Guth,Beatriz Ernestina Cabilio. |
| This study aims to assess the prevalence of Shiga toxin-producing Escherichia coli (STEC) in ground beef collected in two cities located in the State of São Paulo, Brazil. A total of 250 samples of raw ground beef were collected in local grocery stores during the period of March to December 2002 in the cities of Ribeirão Preto (114 samples) and Campinas (136 samples), São Paulo State, Brazil. The samples were processed according to standard methods. The resulting 591 E.coli colonies were screened for STEC by hybridization assays using the specific DNA probes, stx1,stx2 and eae. Further characterization of STEC isolates included the search for the ehxA sequence, detection of enterohemolysin and expression of Shiga toxin using the Vero cell assay. STEC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Shiga toxin; STEC; Serotypes; Ground beef. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300032 |
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| Koochakzadeh,A; Askari Badouei,M; Zahraei Salehi,T; Aghasharif,S; Soltani,M; Ehsan,MR. |
| ABSTRACT The aim of this study was to investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains and to identify the stx gene types in wild captive and companion birds. In total,657 E. coli isolates from 219 birds belonging to 38 different species were investigated for the presence of STEC and EPEC strains. It was shown that five birds (2.28%) carried strains positive for one or more of the virulence factors investigated. The results indicated that 1.8% (n=4) and 0.45% (n=1) of the birds carried STEC and EPEC strains, respectively. All STEC strains harbored the stx2f and eae genes and this finding reveals the role of other birds, in addition to pigeons, as reservoirs of STEC. The only EPEC strain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EPEC; STEC; Stx2f; Pet birds; Wild birds. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400445 |
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| Caliman,M.W.; Marin,J.M.. |
| Determinadas linhagens de Escherichia coli comportam-se como patógenos em gatos, causando doenças gastrointestinais e extraintestinais. Neste estudo, foram utilizadas 205 cepas de E. coli isoladas de amostras de fezes provenientes de 19 gatos diarreicos e de 21 gatos não diarreicos, e três amostras de urina provenientes de gatos com infecção do trato urinário (ITU). Essas cepas foram testadas pela técnica de reação em cadeia da polimerase com múltiplos iniciadores para a detecção da presença de genes codificadores de adesinas (pap, sfa e afa), assim como para a detecção dos genes produtores da toxina Shiga-like (stx1 e stx2) e do gene da intimina (eae). Oitenta e dois isolados possuíam genes codificadores de adesinas, dos quais 11 apresentaram o gene pap,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Pet; Cat; ExPEC; STEC; Adhesins; Virulence gene. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401287 |
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| Registros recuperados: 20 | |
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